In a news release, police said Koby Clemens, Mark Ori and Jimmy Goethals were arrested after police were called to the parking lot of a restaurant.
Clemens and Ori were charged with disorderly conduct. Goethals was charged with assault and battery.
All three were held in the Roanoke County-Salem Jail before being released on bond.
The three play for the Salem Avalanche, which played its final game of the season Monday in Lynchburg.
In a statement, the Houston Astros said they were attempting to sort out the circumstances leading to arrest of the three players in their farm system.
"Based on the feedback and information we get from the court appearance, we will make a decision as to what disciplinary action will be taken," said Ricky Bennett, assistant general manager/director of player development for the Astros.
The police statement said 50 people were in the parking lot of Mac and Bob's restaurant when officers arrived at 2 a.m. Sunday. The owner of the restaurant did not immediately return a telephone message left by The Associated Press.
Initial reports of the arrests were by broadcast and print outlets in the Roanoke area.
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